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late 15th–early 16th centuries), and are known only through oral history and through archaeological investigations.Others were contemporary with the contact and colonization period, and were documented in historical accounts of the time.But, the DNA was ancestral to present-day South American and Central American Native American populations.The implication is that there was an early divergence between North American indigenous peoples and those of Central and South America.

"Pre-Columbian" is used especially often in the context of discussing the pre-contact Mesoamerican indigenous societies: Olmec; Toltec; Teotihuacano' Zapotec; Mixtec; Aztec and Maya civilizations; and the complex cultures of the Andes: Inca Empire, Moche culture, Muisca Confederation, and Cañari.

These are the Anzick-1 remains from the Anzick Clovis burial in Montana.

The data indicate that the individual was closely related to present North American Native American populations.

Archaeologists and anthropologists have studied differences among these crafted lithic flaked tools to classify cultural periods.), equivalent to 13,500 to 13,000 calendar years ago.

In 2014, the autosomal DNA was sequenced of a 12,500 -year-old infant from Montana, whose remains were found in close association with several Clovis artifacts.

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They also show that the basal northern and southern Native American branches, to which all other indigenous Americans belong, diverged around 16,000 years ago.